Coding

Part:BBa_K2574000:Design

Designed by: Jonathan Tello   Group: iGEM18_Hawaii   (2018-10-09)


VLP forming Gag Construct from Maize CR element.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our design contains a Histidine tag at the 5' end of the sequence that can be used for DNA purification using a nickel based column. This was crucial for further experimentation as we needed pure capsid proteins that will form into VLPs.


Source

Maize genomic sequence.

References